| A cytodetection test of diarrhoeic phycotoxins was developped by both IFREMER and Nantes University. Its principle is based on cell morphological changes of KB strain, induced by inhibition of protein-phosphatases involved in cytoskeleton building. This test has been shown sensitive, fast and specific for protein phosphatase inhibitors like diarrhoeic toxins (specialy okadaic acid in France). It shows a high repeatability and reproducibility with pure toxin for the cell strain is highly stable (minimal active concentration or MAC = 125 ng OA.ml-1) In order to evaluate the efficiency oftransfering.the method to coastallaboratories, the assay was subjected to a prevalidation study. The sampling, consisting of five species of shellfish, was carried out during... |
